Cloning and expression analysis of a wood-associated xylosidase gene (PtaBXL1) in poplar tension wood.
Identifieur interne : 003683 ( Main/Exploration ); précédent : 003682; suivant : 003684Cloning and expression analysis of a wood-associated xylosidase gene (PtaBXL1) in poplar tension wood.
Auteurs : Raphael Decou [France] ; Sabine Lhernould ; Françoise Laurans ; Elodie Sulpice ; Jean-Charles Leplé ; Annabelle Déjardin ; Gilles Pilate ; Guy CostaSource :
- Phytochemistry [ 0031-9422 ] ; 2009.
Descripteurs français
- KwdFr :
- ADN des plantes (génétique), Bois (enzymologie), Bois (génétique), Clonage moléculaire (MeSH), Données de séquences moléculaires (MeSH), Expression des gènes (génétique), Masse moléculaire (MeSH), Phylogenèse (MeSH), Populus (enzymologie), Populus (génétique), Régulation de l'expression des gènes codant pour des enzymes (MeSH), Spectrométrie de masse (MeSH), Séquence d'acides aminés (MeSH), Xylosidases (composition chimique), Xylosidases (génétique), Xylosidases (métabolisme).
- MESH :
- composition chimique : Xylosidases.
- enzymologie : Bois, Populus.
- génétique : ADN des plantes, Bois, Expression des gènes, Populus, Xylosidases.
- métabolisme : Xylosidases.
- Clonage moléculaire, Données de séquences moléculaires, Masse moléculaire, Phylogenèse, Régulation de l'expression des gènes codant pour des enzymes, Spectrométrie de masse, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Cloning, Molecular (MeSH), DNA, Plant (genetics), Gene Expression (genetics), Gene Expression Regulation, Enzymologic (MeSH), Mass Spectrometry (MeSH), Molecular Sequence Data (MeSH), Molecular Weight (MeSH), Phylogeny (MeSH), Populus (enzymology), Populus (genetics), Wood (enzymology), Wood (genetics), Xylosidases (chemistry), Xylosidases (genetics), Xylosidases (metabolism).
- MESH :
- chemical , chemistry : Xylosidases.
- chemical , genetics : DNA, Plant, Xylosidases.
- enzymology : Populus, Wood.
- genetics : Gene Expression, Populus, Wood.
- chemical , metabolism : Xylosidases.
- Amino Acid Sequence, Cloning, Molecular, Gene Expression Regulation, Enzymologic, Mass Spectrometry, Molecular Sequence Data, Molecular Weight, Phylogeny.
Abstract
In stems of woody angiosperms responding to mechanical stress, imposed for instance by tilting the stem or formation of a branch, tension wood (TW) forms above the affected part, while anatomically distinct opposite wood (OW) forms below it. In poplar TW the S3 layer of the secondary walls is substituted by a "gelatinous layer" that is almost entirely composed of cellulose and has much lower hemicellulose contents than unstressed wood. However, changes in xylan contents (the predominant hemicelluloses), their interactions with other wall components and the mechanisms involved in TW formation have been little studied. Therefore, in the study reported here we determined the structure and distribution of xylans, cloned the genes encoding the xylan remodeling enzymes beta-xylosidases (PtaBXLi), and examined their expression patterns during tension wood, normal wood and opposite wood xylogenesis in poplar. We confirm that poplar wood xylans are substituted solely by 4-O-methylglucuronic acid in both TW and OW. However, although glucuronoxylans are strongly represented in both primary and secondary layers of OW, no 4-O-methylGlcA xylan was found in G-layers of TW. Four full-length BXL cDNAs encoding putative beta-xylosidases were cloned. One, PtaBXL1, for which xylosidase activity was confirmed by heterologous expression in Escherichia coli, exhibited a wood-specific expression pattern in TW. In conclusion, xylan as PtaBXL1, encoding beta4-xylosidase activity, are down-regulated in TW.
DOI: 10.1016/j.phytochem.2008.12.019
PubMed: 19162284
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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last="Lhernould">Sabine Lhernould</name></author><author><name sortKey="Laurans, Francoise" sort="Laurans, Francoise" uniqKey="Laurans F" first="Françoise" last="Laurans">Françoise Laurans</name></author><author><name sortKey="Sulpice, Elodie" sort="Sulpice, Elodie" uniqKey="Sulpice E" first="Elodie" last="Sulpice">Elodie Sulpice</name></author><author><name sortKey="Leple, Jean Charles" sort="Leple, Jean Charles" uniqKey="Leple J" first="Jean-Charles" last="Leplé">Jean-Charles Leplé</name></author><author><name sortKey="Dejardin, Annabelle" sort="Dejardin, Annabelle" uniqKey="Dejardin A" first="Annabelle" last="Déjardin">Annabelle Déjardin</name></author><author><name sortKey="Pilate, Gilles" sort="Pilate, Gilles" uniqKey="Pilate G" first="Gilles" last="Pilate">Gilles Pilate</name></author><author><name sortKey="Costa, Guy" sort="Costa, Guy" uniqKey="Costa G" first="Guy" last="Costa">Guy Costa</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date 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87060 Limoges, Cédex, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Chimie des Substances Naturelles, Groupe de Glycobiologie Forestière, Faculté des Sciences et Techniques, 123, Avenue Albert Thomas, 87060 Limoges, Cédex</wicri:regionArea><wicri:noRegion>Cédex</wicri:noRegion><wicri:noRegion>Cédex</wicri:noRegion></affiliation></author><author><name sortKey="Lhernould, Sabine" sort="Lhernould, Sabine" uniqKey="Lhernould S" first="Sabine" last="Lhernould">Sabine Lhernould</name></author><author><name sortKey="Laurans, Francoise" sort="Laurans, Francoise" uniqKey="Laurans F" first="Françoise" last="Laurans">Françoise Laurans</name></author><author><name sortKey="Sulpice, Elodie" sort="Sulpice, Elodie" uniqKey="Sulpice E" first="Elodie" last="Sulpice">Elodie Sulpice</name></author><author><name sortKey="Leple, Jean Charles" sort="Leple, Jean Charles" uniqKey="Leple J" first="Jean-Charles" last="Leplé">Jean-Charles Leplé</name></author><author><name sortKey="Dejardin, Annabelle" sort="Dejardin, Annabelle" uniqKey="Dejardin A" first="Annabelle" last="Déjardin">Annabelle Déjardin</name></author><author><name sortKey="Pilate, Gilles" sort="Pilate, Gilles" uniqKey="Pilate G" first="Gilles" last="Pilate">Gilles Pilate</name></author><author><name sortKey="Costa, Guy" sort="Costa, Guy" uniqKey="Costa G" first="Guy" last="Costa">Guy Costa</name></author></analytic><series><title level="j">Phytochemistry</title><idno type="ISSN">0031-9422</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Cloning, Molecular (MeSH)</term><term>DNA, Plant (genetics)</term><term>Gene Expression (genetics)</term><term>Gene Expression Regulation, Enzymologic (MeSH)</term><term>Mass Spectrometry (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Molecular Weight (MeSH)</term><term>Phylogeny (MeSH)</term><term>Populus (enzymology)</term><term>Populus (genetics)</term><term>Wood (enzymology)</term><term>Wood (genetics)</term><term>Xylosidases (chemistry)</term><term>Xylosidases (genetics)</term><term>Xylosidases (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN des plantes (génétique)</term><term>Bois (enzymologie)</term><term>Bois (génétique)</term><term>Clonage moléculaire (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Expression des gènes (génétique)</term><term>Masse moléculaire (MeSH)</term><term>Phylogenèse (MeSH)</term><term>Populus (enzymologie)</term><term>Populus (génétique)</term><term>Régulation de l'expression des gènes codant pour des enzymes (MeSH)</term><term>Spectrométrie de masse (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term><term>Xylosidases (composition chimique)</term><term>Xylosidases (génétique)</term><term>Xylosidases (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Xylosidases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Plant</term><term>Xylosidases</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Xylosidases</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Bois</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Populus</term><term>Wood</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Gene Expression</term><term>Populus</term><term>Wood</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN des plantes</term><term>Bois</term><term>Expression des gènes</term><term>Populus</term><term>Xylosidases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Xylosidases</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Xylosidases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Cloning, Molecular</term><term>Gene Expression Regulation, Enzymologic</term><term>Mass Spectrometry</term><term>Molecular Sequence Data</term><term>Molecular Weight</term><term>Phylogeny</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Masse moléculaire</term><term>Phylogenèse</term><term>Régulation de l'expression des gènes codant pour des enzymes</term><term>Spectrométrie de masse</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In stems of woody angiosperms responding to mechanical stress, imposed for instance by tilting the stem or formation of a branch, tension wood (TW) forms above the affected part, while anatomically distinct opposite wood (OW) forms below it. In poplar TW the S3 layer of the secondary walls is substituted by a "gelatinous layer" that is almost entirely composed of cellulose and has much lower hemicellulose contents than unstressed wood. However, changes in xylan contents (the predominant hemicelluloses), their interactions with other wall components and the mechanisms involved in TW formation have been little studied. Therefore, in the study reported here we determined the structure and distribution of xylans, cloned the genes encoding the xylan remodeling enzymes beta-xylosidases (PtaBXLi), and examined their expression patterns during tension wood, normal wood and opposite wood xylogenesis in poplar. We confirm that poplar wood xylans are substituted solely by 4-O-methylglucuronic acid in both TW and OW. However, although glucuronoxylans are strongly represented in both primary and secondary layers of OW, no 4-O-methylGlcA xylan was found in G-layers of TW. Four full-length BXL cDNAs encoding putative beta-xylosidases were cloned. One, PtaBXL1, for which xylosidase activity was confirmed by heterologous expression in Escherichia coli, exhibited a wood-specific expression pattern in TW. In conclusion, xylan as PtaBXL1, encoding beta4-xylosidase activity, are down-regulated in TW.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19162284</PMID><DateCompleted><Year>2009</Year><Month>04</Month><Day>22</Day></DateCompleted><DateRevised><Year>2009</Year><Month>02</Month><Day>23</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0031-9422</ISSN><JournalIssue CitedMedium="Print"><Volume>70</Volume><Issue>2</Issue><PubDate><Year>2009</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Phytochemistry</Title><ISOAbbreviation>Phytochemistry</ISOAbbreviation></Journal><ArticleTitle>Cloning and expression analysis of a wood-associated xylosidase gene (PtaBXL1) in poplar tension wood.</ArticleTitle><Pagination><MedlinePgn>163-72</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.phytochem.2008.12.019</ELocationID><Abstract><AbstractText>In stems of woody angiosperms responding to mechanical stress, imposed for instance by tilting the stem or formation of a branch, tension wood (TW) forms above the affected part, while anatomically distinct opposite wood (OW) forms below it. In poplar TW the S3 layer of the secondary walls is substituted by a "gelatinous layer" that is almost entirely composed of cellulose and has much lower hemicellulose contents than unstressed wood. However, changes in xylan contents (the predominant hemicelluloses), their interactions with other wall components and the mechanisms involved in TW formation have been little studied. Therefore, in the study reported here we determined the structure and distribution of xylans, cloned the genes encoding the xylan remodeling enzymes beta-xylosidases (PtaBXLi), and examined their expression patterns during tension wood, normal wood and opposite wood xylogenesis in poplar. We confirm that poplar wood xylans are substituted solely by 4-O-methylglucuronic acid in both TW and OW. However, although glucuronoxylans are strongly represented in both primary and secondary layers of OW, no 4-O-methylGlcA xylan was found in G-layers of TW. Four full-length BXL cDNAs encoding putative beta-xylosidases were cloned. One, PtaBXL1, for which xylosidase activity was confirmed by heterologous expression in Escherichia coli, exhibited a wood-specific expression pattern in TW. In conclusion, xylan as PtaBXL1, encoding beta4-xylosidase activity, are down-regulated in TW.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Decou</LastName><ForeName>Raphael</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Laboratoire de Chimie des Substances Naturelles, Groupe de Glycobiologie Forestière, Faculté des Sciences et Techniques, 123, Avenue Albert Thomas, 87060 Limoges, Cédex, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lhernould</LastName><ForeName>Sabine</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Laurans</LastName><ForeName>Françoise</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Sulpice</LastName><ForeName>Elodie</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Leplé</LastName><ForeName>Jean-Charles</ForeName><Initials>JC</Initials></Author><Author ValidYN="Y"><LastName>Déjardin</LastName><ForeName>Annabelle</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Pilate</LastName><ForeName>Gilles</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Costa</LastName><ForeName>Guy</ForeName><Initials>G</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>01</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Phytochemistry</MedlineTA><NlmUniqueID>0151434</NlmUniqueID><ISSNLinking>0031-9422</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018744">DNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.-</RegistryNumber><NameOfSubstance UI="D014995">Xylosidases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018744" MajorTopicYN="N">DNA, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015971" MajorTopicYN="N">Gene Expression Regulation, Enzymologic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013058" MajorTopicYN="N">Mass Spectrometry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008970" MajorTopicYN="N">Molecular Weight</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014934" MajorTopicYN="N">Wood</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014995" MajorTopicYN="N">Xylosidases</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2008</Year><Month>06</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2008</Year><Month>12</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2008</Year><Month>12</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>1</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>1</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>4</Month><Day>23</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19162284</ArticleId><ArticleId IdType="pii">S0031-9422(08)00609-2</ArticleId><ArticleId IdType="doi">10.1016/j.phytochem.2008.12.019</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country></list><tree><noCountry><name sortKey="Costa, Guy" sort="Costa, Guy" uniqKey="Costa G" first="Guy" last="Costa">Guy Costa</name><name sortKey="Dejardin, Annabelle" sort="Dejardin, Annabelle" uniqKey="Dejardin A" first="Annabelle" last="Déjardin">Annabelle Déjardin</name><name sortKey="Laurans, Francoise" sort="Laurans, Francoise" uniqKey="Laurans F" first="Françoise" last="Laurans">Françoise Laurans</name><name sortKey="Leple, Jean Charles" sort="Leple, Jean Charles" uniqKey="Leple J" first="Jean-Charles" last="Leplé">Jean-Charles Leplé</name><name sortKey="Lhernould, Sabine" sort="Lhernould, Sabine" uniqKey="Lhernould S" first="Sabine" last="Lhernould">Sabine Lhernould</name><name sortKey="Pilate, Gilles" sort="Pilate, Gilles" uniqKey="Pilate G" first="Gilles" last="Pilate">Gilles Pilate</name><name sortKey="Sulpice, Elodie" sort="Sulpice, Elodie" uniqKey="Sulpice E" first="Elodie" last="Sulpice">Elodie Sulpice</name></noCountry><country name="France"><noRegion><name sortKey="Decou, Raphael" sort="Decou, Raphael" uniqKey="Decou R" first="Raphael" last="Decou">Raphael Decou</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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